Journal: Infection and Immunity
Article Title: Interaction of Gardnerella vaginalis and Vaginolysin with the Apical versus Basolateral Face of a Three-Dimensional Model of Vaginal Epithelium
doi: 10.1128/IAI.00646-18
Figure Lengend Snippet: CD59 exhibits higher intensity on basolateral cells of EpiVaginal tissues. Shown is immunohistochemical staining of untreated EpiVaginal tissue. (A) DAB- and hematoxylin-stained tissue. (B) Quantification of average DAB staining intensity per cell. Data exhibited a normal distribution and equal variance. ***, P ≤ 0.001 (n ≥ 3). Error bars represent standard deviations.
Article Snippet: Immunohistochemistry (IHC) staining for CD59 (1:100; Novus Biologicals) was performed in the CMMCL with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (EDTA, pH 8.0; Leica).
Techniques: Immunohistochemical staining, Staining